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. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: Methods Mol Biol. 2012;839:133–146. doi: 10.1007/978-1-61779-510-7_11

Fig. 3. Dissection and assessment of embryonic length to width ratio after culture.

Fig. 3

(A) Healthy embryos at early somite stage, following 18-20 h in culture. (B, B’) Cultured embryo viewed from the ventral surface (rostral to upper left). Arrow in B’ indicates DiI-labelled cells extending along the midline. (C,D) Removal of the yolk sac and ectoplacental cone prior to analysis in cultured embryos. (E) Flat mounted wild type embryo showing the method for measuring length to width ratio. Scale bars represent: 400 μm in A, 200 μm in B-E.