Table 1.
Comparative Cell Cycle Analysis of Pluripotent Stem Cells
| Cell line | % G1 | % S | % G2 |
|---|---|---|---|
| J1 | 17.6 | 60.1 | 17.1 |
| mESC1 | 11.1 | 65.2 | 16.5 |
| mESC2 | 8.3 | 63.7 | 15.2 |
| Mean±SEM | 12.3±2.75 | 63.0±1.51 | 16.3±0.56 |
| miPS1 | 22.2 | 45.8 | 28.9 |
| miPS2 | 16.5 | 57.1 | 23 |
| miPS3 | 19.7 | 53.1 | 25.0 |
| Mean±SEM | 19.5±1.65 | 52.0±3.31 | 25.6±1.73 |
| FMR1 | 23.1 | 44.7 | 27.9 |
| FMR2 | 17.4 | 60.0 | 19.8 |
| FMR3 | 15.2 | 58.6 | 21.9 |
| Mean±SEM | 18.6±2.35 | 54.4±4.88 | 21.9±2.43 |
Gating for GFP+ cells was as described. Propidium iodide–based fluorescence was measured after compensation for GFP fluorescence spillover in the Fl2-A (APC) channel. A total of 50,000 events were recorded. The population sizes in the respective cell cycle stages were estimated using the Jett–Dean algorithm for cell cycle modeling.
ESC, embryonic stem cells; SEM, standard error of the mean; FMR, fusion-mediated reprogramming; GFP, green fluorescent protein.