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Boc-Xaa-ONp hydrolysis by HCMV A143T/A144T protease mutant. 50 μM Boc-Xaa-ONp (Xaa represents amino acids Phe, Leu, Ile, Ala, Val, Gln, Gly) and Boc-l-Gly-l-Gly-ONp were incubated with 10 μg/mL HCMV A143T/A144T protease mutant for up to 10 min at 30 °C. p-Nitrophenol generated by the reaction was detected at 405 nm (n = 3).
