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Ganciclovir prodrug hydrolysis by HCMV A143T/A144T protease mutant. Ganciclovir prodrugs were subjected to hydrolysis by HCMV protease A143T/A144T mutant at 30 °C. The resulting samples were analyzed by HPLC, and the concentrations of prodrugs were determined. Data are expressed as mean specific activity ± SE (n = 6).
