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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Cell Biol. 2012 Oct 14;14(11):1192–1202. doi: 10.1038/ncb2595

Figure 3. E2f8 is sufficient to promote hepatocyte endocycles.

Figure 3

a, Representative H&E sections of aged (12mo) livers. Scale bar, 12.5µm. b, Quantitative RT-PCR analysis of E2f7 and E2f8 in pre- (E13.5) and post-natal (P0-12mo) wild type livers, with n per time point analyzed as indicated. 3wk*, 1 day post weaning (3wk). c, Left graph, assessment of nuclei volume by confocal imaging and 3-D reconstruction in 6-month-old control and Alb-78dko hepatocytes. n, number of nuclei evaluated per genotype. Right panels, representative confocal images of DAPI-stained liver sections. Scale bar, 10µm. d, Assessment of hepatocyte size in young (3wk), adult (2-6mo) and aged (12mo) livers by quantifying number of hepatocytes per image field, with n per genetic group analyzed as indicated. e, Left graph, quantification of binucleated hepatocytes in young, adult and aged livers, with n per genetic group analyzed as indicated. Right panels, immunofluoresence with anti-E-cadherin marking the boundary of mono- or binucleated hepatocytes. Scale bar, 10µm. f, Left column, flow cytometry of liver nuclei in young, adult and aged livers. n, number of livers analyzed per genotype and age group. Right column, representative FACS profiles of liver nuclei from aged (12mo) mice. g, Immunohistochemistry and quantification of hepatocyte proliferation in 2-month-old livers, with n per genetic group analyzed as indicated. Scale bar, 10µm. control, E2f7f/f;E2f8f/f; Alb-7ko, Alb-cre;E2f7f/f; Alb-8ko, Alb-cre;E2f8f/f; Alb-78dko, Alb-cre;E2f7f/f;E2f8f/f. FACS, fluorescence activated cell sorting. Data in b–g reported as average values, ± SD are included when n>2 samples were analyzed. One-way ANOVA, * p≤0.05; ** p≤0.01; ***p≤0.001.