Figure 7.

Pre-treatment with anti-SR-A and anti-SREC-I blocking antibodies enhances hepatocyte transduction efficiency. Wild-type BALB/c mice were injected with anti-SR-A Fab (SR-A), anti-SREC-I Fab (SREC-I), a combination of both (SR-A + SREC-I), unrelated IgG Fab (unrelated Fab), or saline 5 minutes before the injection of 5 × 10¹¹ vp/kg of HDAd-AFP. Pre-treatments with anti-SR-A or anti-SREC-I Fabs and with a combination of both resulted in increased serum AFP levels at 7 days post-injection (at least n = 7 per treatment group; one-way ANOVA and post-hoc multicomparison Tukey's test: *P < 0.05; **P < 0.01 compared with saline group; ^#P < 0.05 compared with unrelated Fab). AFP, α-fetoprotein; ANOVA, analysis of variance; HdAd, helper-dependent adenoviral vector; vp, viral particle.