Figure 1.

Type I interferon (IFN) mRNA is induced in livers at 1 hour post-injection of HDAdLacZand amplifies cytokine expression in livers and blood of mice. Wild-type (WT) C57Bl/6 mice were injected with 5 × 10¹¹ viral particle (Vp)/kg of HDAdLacZ into tail veins. (a) Total RNA was extracted from livers at 1 hour post-injection, and the levels of IFNβ and IFNγ mRNA were determined by quantitative real-time PCR. Each value was calculated relative to that of control mice injected with phosphate-buffered saline (PBS). Data are presented as means ± SD (n = 3). *P < 0.005. The experiment was repeated with similar results. WT C57Bl/6 and IFNαR^−/− mice were injected with 5 × 10¹¹ Vp/kg of HDAdLacZ into tail veins. (b) Total RNA was extracted from livers at 1, 3, 6, 12, and 24 hours post-injection, and the levels of IFNβ, IL-6, and MCP-1 mRNA were determined by quantitative real-time PCR. Each value was calculated relative to that of control mice (untreated). Data are presented as means ± SD (n = 3). *P < 0.002 and **P < 0.01 for IFNβ. *P < 0.001 and **P < 0.01 for IL-6. *P < 0.003 and **P < 0.001 for MCP-1. (c) Plasma samples were collected after 1, 3, 6, 12, and 24 hours post-injection. IL-6 and MCP-1 levels were measured by using BD cytokine multiplex bead array system. Data are presented as means ± SD (n = 5). *P < 0.01 and **P < 0.05 for IL-6. *P < 0.001 for MCP-1. HDAd, Helper-dependent adenoviral vector.