Figure 4.

Coadministration of MS-275 with oncolytic vaccine booster generates more functional tumor antigen-specific T cells. Blood-derived DCT_180–188-specific CD8⁺ T cells were obtained 5 days after VSV boosting and their cytokine production and avidity were assessed. (a) The frequency capable of simultaneous production of IFN-γ and TNF-α and the amount of (b) IFN-γ and (c) TNF-α produced per cell (measured as mean fluorescence intensity). (d) The functional avidity of DCT_180–188-specific CD8⁺ splenocytes was assessed by flow cytometric detection of intracellular IFN-γ after in vitro restimulation with different dilutions of cognate peptide (n = 5 for each treatment). DCT, dopachrome tautomerase; IFN, interferon; TNF-α, tumor necrosis factor-α VSV, vesicular stomatitis virus.