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. Author manuscript; available in PMC: 2013 Oct 3.
Published in final edited form as: J Am Chem Soc. 2012 Sep 19;134(39):16401–16412. doi: 10.1021/ja307276z

Figure 6.

Figure 6

Evidence that 1:1 Cu(I) to protein species are mononuclear. Top panel, comparison of Fourier transforms of the Se EXAFS of the M160SeM species at Cu(I) to protein ratios of 1:1 and 2:1 showing near equivalency of the intensity of the Se-Cu peaks in the FT expected for a mononuclear Cu(I) species. Bottom panel, absorption edges of Cu(I) mononuclear species compared with the 3-coordinate Cu(I) derivative of the H135A variant of B. subtilis Sco (where Cu(I) is bound by 2 Cys and 1 Met residue). The low intensity of the 8983 eV peaks of the mononuclear Cu(I) CuA intermediates suggest 4-coordination.