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. Author manuscript; available in PMC: 2013 Oct 3.
Published in final edited form as: J Am Chem Soc. 2012 Sep 19;134(39):16401–16412. doi: 10.1021/ja307276z

Table 2.

Rate constants for the individual reaction steps leading to the formation of the mixed-valence form of M160SeM T. thermophilus CuA.

Reaction Stepa Rate Constant
A + Cu(II) → Iearly 7.5±1.1 × 103 mol−1s−1
Iearly → I 0.81±0.18 s−1
I + Cu(II) → A(S-S) + 2Cu(I) 3.0±0.31 × 103 mol−1s−1
I + Cu(I) → P 11.0±3.8 × 106 mol−1s−1
A + Cu(I) → I-Cu(I) indeterminate
a

A is the bis-thiol apo protein, A(S-S) is the disulfide bridged apo protein, P is the mixed valence product, Iearly is the red copper intermediate, I is the green copper intermediate, and I-Cu(I) the mononuclear Cu(I) intermediate. I-Cu(I) may react with Cu(I) to form the di-Cu(I) product but no information on this step could be extracted from the data. The rate constant for reaction of A with Cu(I) is also not well defined.