Fig. 3.
PTEN expression is induced and proliferation is inhibited in early Apccko lesions. Expression of PTEN (representative of n = 3 in each group) in juvenile control (A) and mutant ovaries (B) by IHC. Colocalization of β-catenin and PTEN in juvenile control (C and D) and mutant ovaries (E and F) by IF. Coimmunostaining of β-catenin and pH3 in juvenile control (G) and mutant (H) ovaries. PTEN protein expression in adult control (I) and mutant (J and K) ovaries by IHC. IF for β-catenin and pH3 in adult control (L) and mutant (M) ovaries. Arrowheads indicate OSE in A and L; arrows in B, E, F and H indicate precancerous lesions. Bars: 50 µm.