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. 2013 Feb 5;41(6):3760–3771. doi: 10.1093/nar/gkt058

Figure 3.

Figure 3.

The effect of miR-10a* on the biosynthesis of EGFP-CVB3. (A) MiR-10a* and miR-mock were separately transfected into HeLa cells. The transfected cells were infected with EGFP-CVB3 (MOI = 0.01) 24 h posttransfection. Hoechst 33342 was added to the culture medium to stain the nuclei at 12 h p.i. The EGFP expression in these cells was analyzed at 24 h p.i. (B) The EGFP-positive cell counts were first normalized to the number of nuclei in each sample. The relative counts of EGFP-positive cells of miR-10a*-treated groups were calculated by normalizing to the miR-mock-treated groups. Error bars represent the SDs (n = 6). (C) The EGFP intensity was first normalized to the Hoechst 33342 intensity of the same sample. The normalized EGFP intensities in the miR-10a*-treated cells were further normalized to that in the miR-mock-treated cells. Error bars represent the SDs (n = 6).