Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 8;41(6):3688–3698. doi: 10.1093/nar/gkt068

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — All shRNAs were tested against replicating FL-J6/JFH-Luc HCV in a transient-replication model (A), a stable-replication model (B), as well as against HCVcc infection (C) in Huh7.5 cells. Upper portions of panels A–C show graphic representation of each experimental design. The number corresponding to each shRNA represents the position of the first nucleotide in the recombinant HCV J6(5′UTR-NS2)/JFH1 (GenBank: JF343782.1). The shRNA-SCR was used as negative control and was assigned a value of 100. In (A) and (B), FL-J6/JFH-Luc luciferase levels were normalized to AAV-shRNA encoded eGFP to account for transduction efficiency, whereas in (C), GAPDH quantitative PCR levels and AAV-shRNA eGFP levels were used to normalize the HCV RNA levels measured by quantitative PCR. Error bars represent SD from n = 3–6.