Table 2.

Hydroxybenzoate ligand dissociation constants

Ligand	Abbreviation	PcaV	Kd (μM)
Protocatechuate	PCA	WT	0.67 ± < 0.01
3,5-dihydroxybenzoate	3,5-DHB	WT	4.34 ± 0.52
3-hydroxybenzoate	3-HB	WT	5.91 ± 0.54
4-hydroxybenzoate	4-HB	WT	141.25 ± 1.41
2,5-dihydroxybenzoate	2,5-HB	WT	146.86 ± 1.83
Protocatechuate	PCA	R15A	No binding
Protocatechuate	PCA	R15K	No binding