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. 2013 Feb 4;41(6):3748–3759. doi: 10.1093/nar/gkt040

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Self-assembly experiments using tectoRNA. (A) The secondary structure of tectoRNA, a self-assembling RNA consisting of fluorescently labelled R(11 nt)_Loop (right) and unlabelled L(GAAA)_Receptor (left) RNAs. The two RNAs assembled via GAAA/R(11 nt) as a common clamp (upper dashed line) and other tetraloop/receptor interactions as analyte modules (lower dashed line). (B) Representatives of PAGE analysis of assembling tectoRNAs. Upper and lower bands represent the hetero dimer and dye-labelled monomer, respectively. The mobility of the dimer band of R(11 nt)_L(GUAA) + L(GAAA)_R(B7.8) varied continuously with concentration of unlabelled RNA, indicating a fast exchange kinetics between monomer and dimer forms. Assembling was performed as described in the Methods section.