Figure 3.

Characterization of pyrvinium pamoate action. (A) Chemical structure of pyrvinium pamoate. (B) Titration curve of pyrvinium pamoate using cells transiently transfected with the HTR2c reporter construct. The changes in exon Vb usage were determined using the primers schematically indicated in Figure 1. The graph underneath shows the statistical evaluation of three independent experiments. (C) Time course of pyrvinium pamoate using an assay similar to the one in (B). (D) Effect of pyrvinium pamoate on exon Vb inclusion in the presence of cycloheximide. Cells were transiently transfected with the HTR2c gene and treated with 10 µM cyclohexamide overnight. The cells were then treated with pyrvinium pamoate for 2 h.