Figure 7.

Pyrvinium pamoate increases snRNA binding to HTR2c exon Vb. (A) Schematic outline of the method used to detect U1snRNA and exon 5B binding. The biotinylated probe corresponds to the RNA used in Figure 6. (B) RT–PCR analysis of RNA extracted from streptavidin-conjugated Dynabeads. Lane 1 and 2: RNA probe folded in DMSO; lane 3 and 4, RNA probe folded in 10 µM pyrvinium pamoate; lane 5 and 6: nuclear extract only; lane 2, 4 and 6: no UV cross-linking. (C) Quantification and statistic analysis of the RT–PCR results.