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. 2013 Feb 4;41(6):3874–3887. doi: 10.1093/nar/gkt053

Figure 3.

Figure 3.

Biochemical characterization of the XMRV RT variants. The DNA strand transfer assay is outlined schematically in (A) and described in the main text. The results are shown in (B). Notations P20 and T40 indicate migration positions of the Cy3-labelled, 20 nt DNA primer and Cy5-labelled RNA template, respectively, before initiation of DNA synthesis. Full-length primer extension on the donor RNA template is evidenced by accumulation of the strand transfer intermediate, STI40, whereas RNase H hydrolysis products are defined by R21/20, R18/17 and R14/13. Transfer of nascent DNA to the acceptor RNA template and continued DNA synthesis is evidenced by accumulation of the 60 nucleotide strand transfer product, STP60. Samples were withdrawn after 5 min (Lanes a), 10 min (Lanes b), 20 min (Lanes c) and 40 min (lanes d) for analysis. Positions of Ala substitutions XMRV RT are indicated below each panel and shown in (C) with lime green for K397A/K398A, blue for R311A/K425A and purple for W406A/R456A.