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Two-step one-pot two-enzyme synthesis of fluorinated T-antigens and derivatives using EcGalK and BiGalHexNAcP.
| Donors | Acceptors | Products | Yields (%) |
|---|---|---|---|
| Gal 1 |
 2 GalNAc |
 6 Galβ1–3GalNAc |
81 |
| Gal 1 |
 3 GalNAcαProN3 |
 7 Galβ1–3GalNAcαProN3 |
86 |
| Gal 1 |
 4 GalNAc6FαProN3 |
 8 Galβ1–3GalNAc6FαProN3 |
88 |
| Gal6F 5 |
 2 GalNAc |
 9 Gal6Fβ1–3GalNAc |
80 |
| Gal6F 5 |
 3 GalNAcαProN3 |
 10 Gal6Fβ1–3GalNAcαProN3 |
87 |
| Gal6F 5 |
 4 GalNAc6FαProN3 |
 11 Gal6Fβ1–3GalNAc6FαProN3 |
82 |
