Table 4.
Test of sensitivity of Pan-Flavi assay with pre-quantified viral RNA dilutions
| Quantity (Genome copies/reaction) | Ct values for DENV-1 | Ct values for TBEV strain K23 | Ct values for YFV strain 17D |
|---|---|---|---|
| 1 × 107 |
16.8 |
16.6 |
- |
| 1 × 106 |
20.5 |
19.5 |
20.8 |
| 1 × 105 |
23.4 |
23.0 |
24.2 |
| 1 × 104 |
27.4 |
26.2 |
27.6 |
| 1 × 103 |
30.2 |
29.4 |
30.1 |
| 1 × 102 |
37.1 |
31.7 |
36.4 |
| 1 × 101 | 39.7 | 33.0 | No Ct |
GC: genome copies, Ct: threshold cycle.
YFV: Yellow fever virus, TBE: Tick-borne encephalitis virus, DENV: Dengue virus.