Fig. 3.

Early endosomal disruption in basal forebrain cholinergic neurons. A) BFCNs in the MSN of APP23 and APPLd2 mice were identified using an antibody against ChAT (green), with rab5b immunolabeling used to show early endosomal morphology (red). Quantitation of average endosome size, as described in the Materials and Methods section, is depicted in (B) (average endosome size ± SEM). Individual endosome area from a minimum of 100 cholinergic neurons per genotype was measured (n = 4 each genotype). Labeling conditions and exposure times were identical throughout. Scale bar, 10 μm. Differences from wt were significant at **p < 0.01.