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. 2013 Apr 4;9(4):e1003401. doi: 10.1371/journal.pgen.1003401

Figure 6. BPA exposure altered DNA methylation at the Igf2 DMR1 and H19/Igf2 ICR in the embryos.

Figure 6

A. Pyrosequencing assays tested methylation of 4 and 6 CpGs (highlighted in bold) in 457 bp and 221 bp genomic regions at the Igf2 DMR1 and H19/Igf2 ICR, respectively. Methylation at the (B) Igf2 DMR1 and (C) H19/Igf2 ICR was analyzed in embryos from controls, lower dose and upper dose exposure groups. Analysis in the upper dose exposure group included embryos that showed monoallelic (black circles) or biallelic (red circles) expression of the Igf2 gene. Sample sizes analyzed in each exposure group are indicated with a = P<0.05 and b = P<0.01. Black horizontal line indicates average methylation level in each exposure group.