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. 2013 Apr 4;9(4):e1003450. doi: 10.1371/journal.pgen.1003450

Figure 1. cyfip regulates synapse development.

Figure 1

(A–E) Representative NMJ4 synapses from different genotypes co-stained with anti-HRP recognizing the neuronal plasma membrane (green) and an antibody against DLG (red), a postsynaptic scaffold protein. Arrows indicate interbouton spacing; asterisks denote superboutons with multiple satellite boutons attached. Scale bar, 10 µm. (F–H) Statistical results of NMJ length (F), the number of satellite boutons (G), and the number of superboutons (H) in different genotypes. Ubiquitous expression of cyfip controlled by act-Gal4 fully rescued the NMJ phenotypes, whereas pre- and postsynaptic expression of cyfip by elav-Gal4 and C57-Gal4, respectively, partially rescued the satellite bouton phenotype of cyfip85.1 null mutants. n = 20 for each genotype; * p<0.05, *** p<0.01, *** p<0.001; error bars indicate SEM.