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. 2013 Apr 4;9(4):e1003450. doi: 10.1371/journal.pgen.1003450

Figure 2. Enlarged synaptic vesicles at active zones and more cisternae in the synaptic boutons of cyfip mutants.

Figure 2

(A, B) Electron micrographs of synaptic boutons from wild type (A) and cyfip85.1 mutants (B). Asterisks indicate active zones with T bars. Compared to wild type, cyfip85.1 mutants exhibited significantly more cisternae (arrows in B). Scale bar, 500 nm. (C–E) High magnification view of representative active zones in wild type (C), cyfip85.1 mutants (D), and ubiquitous expression of Cyfip driven by act-Gal4 in cyfip85.1 background (E). Arrowheads in (D) indicate enlarged vesicles near the T-bar. Dashed line defines a 200 nm radius around the active zone for quantitative analysis of SVs. (F, G) Quantification of the number (F) and diameter (G) of SVs within a 200 nm radius of the active zone. n = 446 SVs for wild type, n = 350 SVs for mutants, and n = 445 SVs for the rescue. (H, I) Frequency distributions (H) and cumulative probabilities (I) of SV diameters in the defined area around the active zone.