Figure 7. New F-actin assembly is enhanced in cyfip mutants.

(A–F) Synaptic boutons of wild type (A–C) and cyfip^85.1 mutants (D–F) under different conditions: vehicle-treated control, treated with jasplakinolide, and after 1 h of jasplakinolide washout. The NMJ boutons were double-labeled with anti-HRP (green) and phalloidin (red) to reveals postsynaptic F-actin. (G) Fluorescence recovery index in the NMJ boutons of different genotypes after jasplakinolide treatment. At least 15 different boutons from 4 animals of each genotype were analyzed. (H–J) Phalloidin labeled (red) cyfip mosaic eye discs of third instar larvae under different conditions: vehicle-treated control (H), treated with jasplakinolide (I), and 90 min recovery after jasplakinolide washout (J). cyfip^85.1 mutant clones marked by the absence of GFP and outlined by white dotted lines show enhanced F-actin assembly in a subset of mutant cells (indicated by arrows). (K) F-actin recovery in a wild-type eye disc. Arrowheads indicate MF; horizontal arrow in H points to posterior.