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. 2013 Apr 4;9(4):e1003438. doi: 10.1371/journal.pgen.1003438

Figure 7. Functional analysis of Mlx-regulated genes.

Figure 7

(A) Pupation kinetics of control (tubts-GAL4>) and Cabut RNAi (tubts-GAL4>Cabut RNAi) larvae on 20% yeast diet with or without 15% sucrose. To avoid early larval lethality, we used temperature sensitive GAL80 with tub-GAL4 system to induce RNAi expression during larval stage. Larvae were maintained in +29°C after collection. Cabut knockdown causes a moderate delay also in the low sugar (20% yeast) diet, but addition of 15% substantially reduces survival and delays pupation further. (B) Pupation kinetics of Aldh-III RNAi (tub-GAL4>Aldh-III RNAi) larvae on 20% yeast diet with or without 15% sucrose. (C) Survival of control (tub-GAL4>) and Aldh-III RNAi (tub-GAL4>Aldh-III RNAi) larvae after 6 days on 20% sucrose-only diet. (D) Survival of control, mlx1, tub-GAL4 and mlx1, tub-GAL4>UAS-Aldh-III larvae after 4 days on a 20% sucrose-only diet. (E) Hemolymph glucose levels in control (tubts-GAL4>) and Cabut RNAi (tubts-GAL4>Cabut RNAi) larvae on 20% yeast-5% sucrose. (F) Hemolymph glucose levels in control (tub-GAL4>) and Aldh-III RNAi (tub-GAL4>Aldh-III RNAi) larvae on 20% yeast-5% sucrose. (G) Total pupation of Fas RNAi (tub-GAL4>Fas RNAi) larvae on 20% yeast diet with or without 15% sucrose. (H) Hemolymph glucose levels in control (tub-GAL4>) and PFK2 RNAi (tub-GAL4>PFK2 RNAi) larvae on 20% yeast-5% sucrose. (I) Pupation kinetics of PFK2 RNAi (tub-GAL4>PFK2 RNAi) larvae on 20% yeast diet with or without 15% sucrose. Error bars represent ± SEM (* p<0.05; ** p<0.01; *** p<0.001).