Table 1.
Intron primers used to amplify the SCN5A and GPD1L exons
| Gene/Exon | Forward primer (5′ to 3′) (base pairs) | Reverse primer (5′ to 3′) (base pairs) | Product length (base pairs) | Annealing temperature (°C) |
|---|---|---|---|---|
|
SCN5A |
|
|
|
|
| 2 |
GAA TCA GGC CCA TTG TCT GTG (21) |
GAG TTG CAC AGA AGG GTA GGC (21) |
400 |
59 |
| 3 |
CAC AGT CCA AGG GCT CTG AG (20) |
GAA TCA GCG CTA CTC TCA CTC C (22) |
315 |
59 |
| 4 |
ATG CTG CTC AGC TTT CCT TG (20) |
GGC AAA AGA GGG TAG AAG CTG (21) |
282 |
57 |
| 5 |
ACG TAA GGA ACC TGG AGA ACC (21) |
GCA AGG CAT AGC ACA GCA TAG (21) |
236 |
57 |
| 61 and 72 |
GGT GGT TCT GCT TTG TAA TTC C (22) |
CCC AGG CAT ATC CCT CTA GC (20) |
415 |
57 |
| 8 |
CCG TGC TTG TTC TTG CCT TC (20) |
GTC TGT CCT CTG TCT GGG TCT C (22) |
399 |
59 |
| 9 |
CCC TCA CCA GCA TGA TGT TTC (21) |
CCT GGC AGG TAA GGG AGA CAA (21) |
231 |
59 |
| 10 |
GAC GGC ATG GAA CAA AGT CTC (21) |
CCC ACA CTT GCT GTC CCT TG (20) |
213 |
59 |
| 11 |
CCA GTG AGG GTG ACC TCT GC (20) |
CAG TCA GGT GAG GGC TTA GAG G (22) |
281 |
61 |
| 12 |
GCT GCA CAA AGT CTC AAT GAT G (22) |
CCA CCC TGG AAA AGC TAG AAC (21) |
267 |
57 |
| 13 |
GAA GAG CAC ATG TCA TGG TCG TG (23) |
CAG ACC TGC TGT GGT GCC TG (20) |
456 |
61 |
| 14 |
GCT GAC GCA AAT CTC CTG AT (20) |
GCT TAA ATG ACC TGG GGT TG (20) |
187 |
57 |
| 15 |
CTA ACT CAT TGG CTG TCC CC (20) |
CTT ACC CAT GAA GGC TGT GC (20) |
347 |
57 |
| 16 |
GCC AGG GAG TCT TTC CAT CC (20) |
GTG ATG ACC TCA GAT TGG GTT G (22) |
296 |
59 |
| 17 |
CTT CAC AAG GTC CCC TCC TC (20) |
TGG GTA GAT GGA TTG ATA GAA GG (23) |
488 |
57 |
| 18 |
GGA CTG GAT GGC TTG GCA TG (20) |
GCT CTG GGC CCT GTA TAT GTA GG (23) |
553 |
59 |
| 19 |
GCA TGG GCA GGG TCT GAA AC (20) |
GCT TCA GGG ACA AAG GCA TG (20) |
280 |
59 |
| 20 |
GCT ACT CAG CCC ACA CTC ACA C (22) |
CTC TGG GTG GAA CTG AGG CTA G (22) |
224 |
61 |
| 21 |
CAT TAG ATG TGG GCA TTC ACA G (22) |
CCA GCT GGA GAC CTC CTT TC (20) |
294 |
57 |
| 22 |
GCT TCA TGT CCA CCT TGT CTG (21) |
CAA TGG GTT TCT CCT TCC TG (20) |
252 |
57 |
| 23 |
GCC TAC TGT CTG TCC CCA AC (20) |
GGC CAT AGG ACA TCA GAA GC (20) |
295 |
59 |
| 24 |
GGT CTT GAA AAG GGC ATG TG (20) |
CCA TTG GGA GGA AGG AAG TC (20) |
386 |
57 |
| 25 |
GCT TCT GGC TTC ATC TGT CC (20) |
CAG ACA CTG ATT CCC TGG TG (20) |
228 |
57 |
| 26 |
CCA GCC TGT CTG ATC TCC CTG (21) |
CCA ACA GGG AAG GTG AGA TGG (21) |
283 |
61 |
| 27 |
CAG AAT GAG GTT GGT GCC TTC (21) |
CTG GGC TGA AAG ACT GTG AAG (21) |
246 |
57 |
| 28 |
CCT GCT GAG CAC TTT CCA TTT G (22) |
GGT TGT ACA TGG CAT TCA GCA G (22) |
396 |
57 |
| 29.13 |
GCC ACC AGT AGC CAC AGT CTC (21) |
GGC CTC TGG GTC AAA TTT CTC (21) |
638 |
57 |
| 29.2 |
GTG CCT CTT CCA GAT CAC CAC (21) |
GAT AAC CAT GGC CGA CAC CTC (21) |
619 |
57 |
| 29.3 |
CCT GAG TGA GGA CGA CTT CGA (21) |
GGC TGC TTT TCA GTG TGT CCT (21) |
736 |
57 |
|
GPD1L |
|
|
|
|
| 1 |
ACG GTC CAG GCG GCT ACA TT (20) |
GCT TCT GTG CGC TCA GTA ACG (21) |
364 |
57 |
| 2 |
CTC TCC CGC CCA AGT GAG TT (20) |
CCA AGT GCT GCT CAG CCA AG (20) |
297 |
57 |
| 3 |
CCT TGT GGA CAG GCA AGG TT (20) |
CAA TGT CTA GCC CTG GGC AG (20) |
240 |
57 |
| 4 |
TCC CAT GCC TCT TTG ATT TG (20) |
ACA TCA AAT GTC CAG ACT CCT GG (23) |
243 |
57 |
| 5 |
GGC TGT TAT TAA TAT CCT TGT TGT C (25) |
CAC AGA GCC CAT TCT TGA GGA (21) |
236 |
57 |
| 6 |
GCA TCT GGG CTT TGT CAT CT (20) |
CCA AGC CAG GAG TCT CAA GT (20) |
322 |
53 |
| 7 |
CAG CAT AGC AAG ACC CAT TCT (21) |
GGC TGG AAG TCC TGA GAA TAG (21) |
299 |
53 |
| 8 | CTG AGG TAA ATC CAG TGG CC (20) | CAT CAT GTT GCC AAG TCC TG (20) | 238 | 53 |
1Exon 6 of SCN5A is only present in transcript variant 3, which is represented by the reference sequence NG_008934.1. 2Alternate designation 6, numbering commonly used in the literature. The same reasoning applies to the remaining exons (8 to 29). 3Several pairs of primers were synthesized to polymerase chain reaction-amplify exon SCN5A 29 owing to its large sizes.