TABLE 1.

Dissociation constants (μM) for Binding CaM to PEP-19, PEP(28–62), and PEP(39–62)

Assay	PEP-19	PEP(28–62)	PEP(39–62)
			Kd1	Kd2
NMRa	29 ± 0.7	24 ± 0.8	—b	—
Fluorescencec	18 ± 3	NDd	0.24 ± 0.04	—
FRET	20 ± 4	ND	0.16 ± 0.05	27 ± 3

^aValues are the average mean ± S.E. of K_d values derived separately from chemical shift changes for ¹H and/or ¹⁵N nuclei of amides for residues 99, 105, 109, 116, 117, 146, and 147.

^bNMR data fit poorly to either single or two-site binding models. Fluorescence data fit best to a single-site binding model.

^cThe values derived from fluorescence and FRET data are the mean ± S.E. of three to four experiments.

^dND, not determined.