Skip to main content
. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: J Biol Chem. 2007 Nov 8;283(3):1401–1410. doi: 10.1074/jbc.M703831200

TABLE 3.

Effect of PEP-19, PEP(28–62), and PEP(39–62) on Ca2+ dissociation from CaM

Condition koff rate
(s−1)
CaM 9.9 ± 0.4
CaM-(76–148) 12.1 ± 0.2
CaM + PEP-19 430 ± 50
CaM-(76–148) + PEP-19 490 ± 20
CaM + PEP(28–62) 390 ± 40
CaM + PEP(39–62) 3.2 ± 0.3

Dissociation rates (koff) were derived by fitting data such as that in Fig. 5B to a single exponential equation. The values are the mean ± S.D. of three to six experiments. CaM was present at 2 to 5 μM, and PEP-19 or PEP-19 peptides were present at 30 μM.