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Apoptosis of SH-SY5Y cells treated with different Mn concentrations was assessed using annexin V/PI staining and flow cytometry. Quadrants: lower left: live cells; lower right: early apoptotic cells; upper right: late apoptotic cells and upper left: necrotic cells. Pearson’s correlation test and paired t-test were used for statistical analysis of data from triplicate independent flow cytometry experiments.
