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. 2013 Apr 4;8(4):e60620. doi: 10.1371/journal.pone.0060620

Figure 1. Human BSEP and MDR3 expression in Pichia pastoris.

Figure 1

A 5 µg of membranes derived from P. pastoris cells carrying the empty expression plasmid pSGP18 (neg ctrl), BSEP or BSEP-GFP were subjected to SDS-PAGE and immunoblotting (lanes from left to right). The negative control (left lane) did not react with the monoclonal antibody (F-6), while BSEP (middle lane) and BSEP-GFP (right lane) could be detected by the same antibody. B Identical samples were probed with a monoclonal GFP antibody. The negative control (left lane) as well as BSEP (middle lane) showed no signal with anti-GFP antibody, while BSEP-GFP could be detected (right lane). C In case of MDR3 the negative control (left lane) showed no signal with the monoclonal antibody C219; MDR3 (middle lane) as well as MDR3-GFP (right lane) could be detected with the monoclonal antibody C219. D Identical MDR3 samples were probed with a monoclonal GFP antibody. The negative control (left lane) as well as MDR3 (middle lane) showed no signal with anti-GFP antibody, while MDR3-GFP could be detected (right lane). The position of the molecular weight markers are shown on the left.