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. 2013 Apr 4;8(4):e60567. doi: 10.1371/journal.pone.0060567

Figure 3. Evaluation of BP-induced expression of several endogenous estrogen-regulated genes.

Figure 3

After hormone deprivation for 24 h, MCF-7 cells were grown in medium containing 2.5% dextran-treated charcoal stripped FBS and treated with 10−8 M E2 or different concentrations of BPs (10−8, 10−7 and 10−6 M, illustrated by color gradations) for 48 h. In addition, treatments to 10−6 M BPs were also performed in presence of 10−7 M of the anti-estrogen ICI182,780 (ICI) (open bars for BP + ICI treatments and hatched bar for E2 + ICI treatment). The expression levels of several E2-regulated genes, (A) CXCL12, (B) Amphiregulin, (C) pS2 and (D) Progesterone Receptor (PR), after the treatments were quantified using real-time PCR. Data are the mean values from triplicate experiments ± SEM (* P<0.05, **P<0.01, ***P<0.001).