Figure 8. Th2 in vitro differentiation is not impaired in naïve β5i KO CD4+ T cells.

Total naïve CD4+ cells were isolated by homogenization and subsequent magnetic associated cells sorting (Miltenyi) from spleens and lymphnodes of 6 weeks old mice and differentiated in presence of plate-bound anti-CD3, soluble anti-CD28, anti-IFN-γ and recombinant murine IL-4. The percentages of IL−4+, IL−5+ and IL−13+ of CD4+ T cells were measured by flow cytometry from in vitro differentiated Th2 cells. The data are representative of two independent experiments and represented as the mean ± SEM (n = 5). (+) represents comparison of Wt vs KO groups respectively. ⁺P<0.05.