Figure 4. Maximum likelihood trees depicting the phylogenetic relationships of DENV-1, -2, and -4 isolated in Puerto Rico, 2010.
Each phylogeny was tested with 1,000 bootstrapping cycles. Each taxa label consists of country of origin (PR = Puerto Rico), year of virus isolation, and GenBank accession number. Viruses isolated and sequenced for this investigation are labeled with a black dot. Genotype names were based on previously published phylogenies [32], [33]. All outgroups have been removed. A: Phylogenies were constructed using 29 DENV-1 E gene sequences: seven from Puerto Rico in 2010, and 22 obtained from GenBank to represent the three main genotypes: American-African, South Pacific, and Asian. B: Phylogenies were constructed using 24 DENV-2 E gene sequences: two from Puerto Rico in 2010, and 22 obtained from GenBank to represent the three main genotypes: American-Asian, Cosmopolitan, and Asian II. C: Phylogenies were constructed using 26 DENV-4 E gene sequences: four from Puerto Rico in 2010, and 22 obtained from GenBank to represent the two main genotypes: Indonesian and South East Asian.
