Table 2. Summary Statistics of RNA-Seq Alignment.
# Reads Total (millions) | # Mapped Reads (millions) | Mapped Reads (% of Total) | # Annotated Mapped Reads (millions) | Annotated Mapped Reads (% of Total) | # Annotated Uniquely Mapped Reads (millions) | # Annotated Exactly Mapped Reads (millions) | |
155-1 (NE-45) | 173 | 167 | 96.5 | 113 | 65.3 | 37.7 | 20.5 |
155-2 (RB-07) | 79.6 | 76.4 | 96.0 | 65 | 81.7 | 23.8 | 9.9 |
155-3 (FS-25) | 54.8 | 52.2 | 95.3 | 47.2 | 86.1 | 22.4 | 13.1 |
155-4 (FS-22) | 76.6 | 73.6 | 96.1 | 63.6 | 83.0 | 25.1 | 9.9 |
155-6 (3024l) | 61.6 | 58.0 | 94.2 | 51.4 | 83.4 | 20.8 | 9.7 |
155-7 (0063) | 73.6 | 69.0 | 93.8 | 61.8 | 84.0 | 26.2 | 13.5 |
157-1 (RB-10) | 106 | 99.4 | 93.7 | 81.2 | 76.6 | 32.5 | 13.1 |
157-2 (NE-42) | 114 | 108 | 95.0 | 91.2 | 80.0 | 34.0 | 12.3 |
157-3 (NE-41) | 99.8 | 94.2 | 94.4 | 78.6 | 78.8 | 39.4 | 16.4 |
157-4 (RB-03) | 108 | 102 | 94.4 | 92.2 | 85.4 | 42.5 | 20.6 |
157-5 (FS-21) | 112 | 105 | 93.6 | 95.8 | 85.5 | 30.4 | 12.5 |
157-6 (RB-16) | 148 | 141 | 95.2 | 126.2 | 85.3 | 57.2 | 29.3 |
157-7 (3024r) | 134 | 126 | 93.7 | 102.8 | 76.7 | 34.6 | 14.2 |
157-8 (2970) | 94.0 | 87.6 | 93.2 | 78.8 | 83.8 | 31.8 | 16.0 |
Code 155 designates flow cell A, code 157 designated flow cell C, followed by the lane number. The sample ID is in parentheses. Normal samples are bolded. Annotated mappings result from Bowtie alignment with the Ensembl 63 GTF file. Uniquely mapped reads are mapped to only one region of the genome by the Bowtie aligner, and exactly mapped reads map to only one location and have no mismatches to the reference.