Table 11. Localization of Enzymatic Activity in Bcell.
Cell Fraction | β-glucan | Glucomannan | Galactan | |||
MUC Activity | MUX Activity | MUC Activity | MUX Activity | MUC Activity | MUX Activity | |
Supernatant | - | - | - | - | ++ | + |
Intracellular Soluble | - | - | - | - | + | + |
Intact Cell Wall | ++ | ++ | +++ | ++ | +++ | +++ |
Cell Wall Solubilization Control | - | - | - | - | - | - |
Lysozyme-Solubilized Cell Wall | +++ | ++ | +++ | ++ | +++ | +++ |
Cultures grown and cell walls prepared and assayed as described in Methods. Legend: - no fluorescence; + weak fluorescence, ++ moderate fluorescence, +++ strong fluorescence.