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. 2013 Apr 4;8(4):e59754. doi: 10.1371/journal.pone.0059754

Figure 6. Translocation of predicted T3S proteins.

Figure 6

(A) Cya translocation assays of Salmonella T3S protein candidates. Each construct was transformed into Salmonella SL1344 (Wild-type) and T3SS-deficient SL1344 strain (InvA-mutant). Duplicate was included for each test. Constructs pBADB-CyaA and pBADB-sipC-CyaA were used as negative control (NC) and positive control (PC), respectively. For each construct, Student’s t test was adopted to compare the cAMP level in the target wells co-incubated with the wild-type strain and InvA-mutant strain. Statistically significance was indicated by star (p<0.05). (B) Statistical analysis for Cya translocation assays of yeast T3S protein candidates. Constructs pBADB-CyaA and pBADB-sipC-CyaA were used as negative control (NC) and positive control (PC), respectively.