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. 2013 Apr 4;8(4):e60213. doi: 10.1371/journal.pone.0060213

Figure 3. The effects of Dex on NRCAM, GPR56 and MEOX1 mRNA accumulation.

Figure 3

(A). Top five genes that were up-regulated and top five genes that were down-regulated by Dex in HPCs. In vitro differentiation of HPCs, total RNA isolation and qRT-PCR were described in ‘Materials and Methods. The relative mRNA levels of [+Dex]/[-Dex] are shown for each gene. (B–D). A time-course experiment of Dex’s effects on NRACM, GPR56 and MEOX1 mRNA levels. HPCs were induced to differentiation for two days followed by hormone treatment. Cells were harvested 2, 8, 24, 48 and 72 h after treatment, total RNA isolated and qRT-PCR performed. The relative mRNA levels of NRCAM (B), GPR56 (C) and MEOX1 (D) are normalized by GAPDH mRNA and the value at 2 h (left) or day 1 (right) is set at 1. (E) Dosage-dependent responses of NRCAM, GPR56 and MEOX1 mRNAs to Dex treatment. Differentiating HPCs were treated with vehicle, 10 nM, 100 nM or 1 µM of Dex or VD3, cell harvested 4 h after treatment, total RNA prepared and subjected to qRT-PCR.