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. 2013 Apr 4;8(4):e60160. doi: 10.1371/journal.pone.0060160

Figure 2. Effect of bacterial cell mediated cytotoxicity as measured by LDH Cytotoxicity Assay.

Figure 2

A–C) THP-1 macrophages were infected at indicated multiplicity of infections and time points to measure the LDH release into the supernatant. The amount of LDH released is proportional to the percentage of lysed eukaryotic cells. D) Human granulocytes were infected at indicated multiplicity of infections for 2 h to measure the LDH release into the supernatant. High control corresponds to maximum lysis achieved using 2% of Triton X-100. Uninfected cells served as control. Data shown are the mean ± SD of three independent experiments. Data is considered significant for p values <0.05 (*).