Table 1. Primers and probe in a nested RT-PCR for Japanese encephalitis virus (JEV).

Primers used	Sequence 5′- 3′	Position in JEV genome	Reference
Outer forward (emf1)	TGGATGACSACKGARGAYATG	10099–10119	Pierre et. al (1994)
Outer reverse (vd8)	GGGTCTCCTCTAACCTCTAG	10771–10752	Pierre et. al (1994)
Inner forward	ATCTGGTGYGGYAGTCTCA	10224–10242	Pyke et al. (2004)
Inner reverse	CGCGTAGATGTTCTCAGCCC	10286–10267	Pyke et al. (2004)
Probe	FAM-CGGAACGCGAWCCAGGGCAA-TAMRA	10244–10263	Pyke et al. (2004)