Table 2. Nested RT-PCR results of samples spiked with Japanese encephalitis virus.

Sample	Dilution	Nested RT-PCR
		Positive/runs in total	Mean ct*
JEV diluted 1∶1000 in TRIzol	1∶1	2/2	14.7
Reagent	1∶10	2/2	16.6
	1∶100	3/3	17.2
	1∶1 000	3/3	21.2
	1∶10 000	2/2	26.3
	1∶100 000	2/2	28.6
JEV diluted 1∶1000 in	1∶1	0/3
homogenate of 5 mosquitoes	1∶10	2/3	34.0
in TRIzol Reagent	1∶100	3/3	22.0
	1∶1 000	2/3	23.2
	1∶10 000	3/3	31.2
	1∶100 000	1/3	34.2
JEV diluted 1∶1000 in	1∶1	0/2
homogenate of 50	1∶10	0/2
mosquitoes in TRIzol	1∶100	2/2	20.6
Reagent	1∶1 000	2/2	24.6
	1∶10 000	2/2	29.6
	1∶100 000	2/2	36.4

^*Mean cycle threshold for the positive runs.