Figure 4. CD160Ig reduces allospecific Th1 cytokine generation in CD28^−/− and CD4^−/− mice.

Heart grafts from BALB/c donors were transplanted into C57BL/6 WT, CD28^−/− or CD4^−/− recipients, which were treated with CD160Ig +/− CTLA4Ig or control Ig. Recipient splenocytes were isolated 10 days (WT recipients) or 14 days (CD28^−/− and CD4^−/− recipients, CTLA4Ig treated WT recipients) after transplantation, cultured with irradiated donor splenocytes. A) The frequency of alloreactive IFN-γ and IL-5 producing T cells was analyzed by ELISPOT. B). The concentration of TNF-α, IL-6, IFN-γ, IL-17, IL-4 and IL-5 was examined by Luminex Assay in the supernatants of the cultures with CD28^−/− responder cells. The data shown are pooled from 3–5 independent experiments performed in quadruplicate wells (Mean±SEM).