Figure 6. Bioluminescence images of Ins1-luc BAC transgenic mice with combined Pdx1, NeuroD, and MafA gene transfer in the liver.

(A) Representative images of mice before (day 0) and after the gene transfer. Designated days indicate days after the infection. Each image is optimally adjusted using Living Image software because a huge difference in luminescence from the pancreas and the liver disables showing images with the same longitudinal photon scale. (B) Bioluminescence images of extracted organs from Ins1-luc BAC transgenic mice 3 days after infection. L, P, and S indicate the liver, pancreas, and spleen, respectively. (C) Immunohistochemistry for anti-insulin antibody of the liver from a WT mouse 3 days after infection. Scale bar: 50 µm. Arrows indicate insulin-positive cells.