Figure 1. Absence of function of KCC3-E289G mutant cDNA.

A) Conservation of glutamic acid residue (E289 in mouse KCC3) within mouse SLC12A cotransporters. The residue, highlighted by an arrowhead in cartoon and sequence alignment, is localized at the end or right downstream of transmembrane domain 3 (TM3). B) K⁺ influx was measured through unidirectional ⁸⁶Rb tracer uptake under isosmotic and hyposmotic conditions, in oocytes injected with water, KCC3-E289G mutant cRNA, and wild-type KCC3 cRNA. Bars represent means±SEM (n = 25 oocytes).