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A) Western blot analysis of wild-type KCC3 and KCC3-E289G mutant in HEK 293FT cells treated with tunicamycin (10 µg/ml for 18 h). B) Western blot analysis of wild-type KCC3 and KCC3-E289G mutant proteins isolated from Xenopus laevis oocytes and treated with PNGase (0.25U, 12 h at 37°C). The membranes were exposed to a rabbit polyclonal anti-KCC3 antibody. The experiment was repeated once with identical data.
