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. 2013 Feb 12;288(14):10035–10050. doi: 10.1074/jbc.M112.436162

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 10. — MS² of the oligosaccharides derived from fraction 181:IV from human embryonic stem cell line SA181, and from reference glycosphingolipids. RT, retention time. A, MS² spectrum of the ion at m/z 868 of fraction 181:IV from human embryonic stem cell line SA181 (retention time 15.1 min). B, MS² spectrum of the ion at m/z 998 of fraction 181:IV from human embryonic stem cell line SA181 (retention time 18.2 min). C, MS² spectrum of the ion at m/z 998 of reference Le^y hexasaccharide, Le^y-6 (retention time 18.2 min). D, MS² spectrum of the ion at m/z 1014 of fraction 181:IV from human embryonic stem cell line SA181 (retention time 16.7 min). E, MS² spectrum of the ion at m/z 1014 of reference Globo H hexasaccharide, Globo H (retention time 16.6 min). F, MS² spectrum of the ion at m/z 1055 of fraction 181:IV from human embryonic stem cell line SA181 (retention time 17.0 min). G, MS² spectrum of the ion at m/z 1055 of reference A type 1 hexasaccharide, A6-1 (retention time 16.9 min). H, interpretation formulas.