FIGURE 11.

The H1/H2 region of the DQF-COSY proton NMR spectrum of fraction 181:IV from human embryonic stem cell line SA181, recorded at 600 MHz (30 °C). The spectrum at the top is the corresponding one-dimensional spectrum of the anomeric region. The sample (∼0.4 mg) was dissolved in dimethyl sulfoxide-D₂O (98:2, v/v) after deuterium exchange. All labeled connectivities represent H1/H2 cross-peaks, except for Galα4 H5/H6a cross-peaks, which are denoted as such. The H1/H2 labels are derived from structures 5–10, and Roman numerals I-VI denote individual sugars, as listed in Table 3.