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. 2013 Feb 12;288(14):10035–10050. doi: 10.1074/jbc.M112.436162

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — LC-ESI/MS of the oligosaccharides obtained by digestion of fraction 181:IV from human embryonic stem cells and of reference glycosphingolipids with Rhodococcus endoglycoceramidase II. A, base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction 181:IV from human embryonic stem cell line SA181. B, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from fraction 181:IV from human embryonic stem cell line SA181. C, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from reference globotetraosylceramide (Gb4; GalNAcβ3Galα4Galβ4Glcβ1Cer). D, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from reference isoglobotetraosylceramide (iGb4; GalNAcβ3Galα3Galβ4Glcβ1Cer). E, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from reference lactotetraosylceramide (L4; Galβ3GlcNAcβ3Galβ4Glcβ1Cer). F, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from reference neo-lactotetraosylceramide (nL4; Galβ4GlcNAcβ3Galβ4Glcβ1Cer). G, mass chromatogram of m/z 706 from LC-ESI/MS of the oligosaccharides derived from reference gangliotetraosylceramide (GgO4; Galβ3GalNAcβ4Galβ4Glcβ1Cer). The retention times of the molecular ions are given in italic type.