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. 2013 Feb 12;288(14):10035–10050. doi: 10.1074/jbc.M112.436162

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — LC-ESI/MS of the oligosaccharides obtained by digestion of fraction 181:IV from human embryonic stem cell line SA181 and of reference glycosphingolipids with Rhodococcus endoglycoceramidase II. A, mass chromatogram of m/z 852 from LC-ESI/MS of the oligosaccharides derived from fraction 181:IV from human embryonic stem cell line SA181. B, mass chromatogram of m/z 852 from LC-ESI/MS of the oligosaccharides derived from reference H type 1 pentaglycosylceramide (H5-1; Fucα2Galβ3GlcNAcβ3Galβ4Glcβ1Cer). C, mass chromatogram of m/z 852 from LC-ESI/MS of the oligosaccharides derived from reference H type 2 pentaglycosylceramide (H5-2; Fucα2Galβ4GlcNAcβ3Galβ4Glcβ1Cer). D, mass chromatogram of m/z 852 from LC-ESI/MS of the oligosaccharides derived from reference Le^a pentaglycosylceramide (Le^a-5; Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer). E, mass chromatogram of m/z 852 from LC-ESI/MS of the oligosaccharides derived from reference Le^x pentaglycosylceramide (Le^x-5; Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer). The retention times of the molecular ions are given in italic type.