TABLE 2.
Primer sequences for two-step promoter mutagenesis
| Name (location of mutated CpG)/part of amplicon direction | Sequence (5′ to 3′)a |
|---|---|
| MMP13–214-Mut1 (-14) | |
| 1st half (forward) | TATGTGAGCAAACAGCAGATTAAAA |
| 1st half (reverse) | TTGTCTTTCCaCAGAGATTACCTTT |
| 2nd half (forward) | AAAGGTAATCTCTGtGGAAAGACAA |
| 2nd half (reverse) | AGACATCTCAAAGTATTCAGCATAGGT |
| MMP13–214-Mut2 (−110) | |
| 1st half (forward)b | AATAAATCTCTTTGTTCAGCTCTCTGTTTC |
| 1st half (reverse) | CTTACaTGGCGACTTTTTCTTTTCCCT |
| 2nd half (forward) | AGGGAAAAGAAAAAGTCGCCAtGTAAG |
| 2nd half (reverse)c | AGACATCTCAAAGTATTCAGCATAGGTGAT |
| MMP13–214-Mut3 (−115) | |
| 1st half (forward)b | AATAAATCTCTTTGTTCAGCTCTCTGTTTC |
| 1st half (reverse) | CTTACGTGGCaACTTTTTCTTTTCCCT |
| 2nd half (forward) | AGGGAAAAGAAAAAGTtGCCACGTAAG |
| 2nd half (reverse)c | AGACATCTCAAAGTATTCAGCATAGGTGAT |
| MMP13–214-Mut4 (−135) | |
| 1st half (forward) | ACCCAATAAATAATAAATCTCTTTGTTCAGCTCT |
| 1st half (reverse) | TTCTTTTCCCTCCCaAGTGTGGTTT |
| 2nd half (forward) | AAACCACACTtGGGAGGGAAAAGAA |
| 2nd half (reverse) | GACATCTCAAAGTATTCAGCATAGGTGATG |
| IL1B-521-Mut1 (+13) | |
| 1st half (forward)d | TTGTTTATGTGAGCAAACAGCA |
| 1st half (reverse) | CCTTGTGCCTCaAAGAGGTT |
| 2nd half (forward) | AACCTCTTtGAGGCACAAGG |
| 2nd half (reverse)e | CCAGCCTCACAGACATCTCA |
| IL1B-521-Mut2 (−20) | |
| 1st half (forward)d | TTGTTTATGTGAGCAAACAGCA |
| 1st half (reverse) | CCCTCaCTGTTTTTATGGCTTT |
| 2nd half (forward) | AAAGCCATAAAAACAGtGAGGG |
| 2nd half (reverse)e | CCAGCCTCACAGACATCTCA |
| IL1B-521-Mut3 (−256) | |
| 1st half (forward) | TTTGTTTATGTGAGCAAACAGCAG |
| 1st half (reverse) | TTTTCCTGACAATCaTTGTGCAGT |
| 2nd half (forward) | ACTGCACAAtGATTGTCAGGAAAA |
| 2nd half (reverse) | TGCCATCTTCCAGAGGGTAG |
| IL1B-521-Mut4 (−299) | |
| 1st half (forward) | TATGTGAGCAAACAGCAGATTAAAAG |
| 1st half (reverse) | AATACTGGATTTTCCCACaTTAGAAG |
| 2nd half (forward) | CTTCTAAtGTGGGAAAATCCAGTATT |
| 2nd half (reverse) | ACTAAGCTTAAGATCTGGCTGAAGAG |
a Lowercase letters indicate a mutated base.
b Common primers.
c Common primers.
d Common primers.
e Common primers.